Error on the Cloud

When running Yatsunenko 16S amplicon data (40 gig combo file). Was running on Instance with ~32GB memory, so am now going to up that to the largest possible instance at 68GB:

ubuntu@ip-10-218-81-140:~$ core_qiime_analyses.py -i /home/ubuntu/data_yatsunenko/ps_metagenome_extract/ps_metagenome_combined.fna -o /home/ubuntu/ps_metagenome_coreanalyses_14sept -m qiime_ps_metagenomes_mappingfile.txt -f –suppress_split_libraries -t /home/ubuntu/gg_otus_4feb2011/trees/gg_97_otus_4feb2011.tre -p /home/ubuntu/qiime_parameters_ps_amplicon.txt
ubuntu@ip-10-218-81-140:~$ core_qiime_analyses.py -i /home/ubuntu/data_yatsunenko/yatsunenko/raw_data/16S_amplicon_yatsunenko_combo.fna -o /home/ubuntu/ps_16S_amplicon_coreanalyses_14sept -m /home/ubuntu/qiime_16S_amplicon_mappingfile.txt -f –suppress_split_libraries -t /home/ubuntu/gg_otus_4feb2011/trees/gg_97_otus_4feb2011.tre -p /home/ubuntu/qiime_parameters_ps_amplicon.txt
Traceback (most recent call last):
File “/home/ubuntu/qiime_software/qiime-1.5.0-release/bin/core_qiime_analyses.py”, line 191, in <module>
main()
File “/home/ubuntu/qiime_software/qiime-1.5.0-release/bin/core_qiime_analyses.py”, line 188, in main
status_update_callback=status_update_callback)
File “/home/ubuntu/qiime_software/qiime-1.5.0-release/lib/qiime/workflow.py”, line 1411, in run_core_qiime_analyses
status_update_callback=status_update_callback)
File “/home/ubuntu/qiime_software/qiime-1.5.0-release/lib/qiime/workflow.py”, line 471, in run_pick_otus_through_otu_table
close_logger_on_success=close_logger_on_success)
File “/home/ubuntu/qiime_software/qiime-1.5.0-release/lib/qiime/workflow.py”, line 135, in call_commands_serially
raise WorkflowError, msg
qiime.workflow.WorkflowError:

*** ERROR RAISED DURING STEP: Pick OTUs
Command run was:
/home/ubuntu/qiime_software/python-2.7.1-release/bin/python /home/ubuntu/qiime_software/qiime-1.5.0-release/bin/pick_otus.py -i /home/ubuntu/data_yatsunenko/yatsunenko/raw_data/16S_amplicon_yatsunenko_combo.fna -o /home/ubuntu/ps_16S_amplicon_coreanalyses_14sept/otus//uclust_ref_picked_otus –max_rejects 8 –otu_picking_method uclust_ref –similarity 0.97 –max_cdhit_memory 400 –refseqs_fp /home/ubuntu/gg_otus_4feb2011/rep_set/gg_97_otus_4feb2011.fasta –clustering_algorithm furthest –word_length 8 –max_accepts 1 –stepwords 8 –max_e_value 1e-10
Command returned exit status: 137
Stdout:

Stderr
Killed

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