Evan’s primer designing

In BLAST outputs, these are the rules for primer directions from OCTU alignments:

Simon A (As is) design from right to left <—————*

Simon B (reverse complement) design from left to right *—————->

Dorota A (complement only) design from left to right *————–>

Dorota B (reverse only) design from right to left <————-*

The Following OCTUs had 100% pairwise similarity in BLAST matches:

  • Simon 916(A) = 11051 (B)
  • Simon 346 (A) = 336 (B)
  • Dorota 1615 (A) = 130 (B)
  • Dorota 1227 (A) = 1484 (B)